Journal: PLoS Pathogens

Article Title: Trichoderma -Plant Root Colonization: Escaping Early Plant Defense Responses and Activation of the Antioxidant Machinery for Saline Stress Tolerance

doi: 10.1371/journal.ppat.1003221

Figure Lengend Snippet: (A) RNA was extracted from untreated Arabidopsis roots and from roots collected from plants exposed to 100 mM NaCl (NaCl), and from roots colonized by T203 for 48 hours prior exposure to 100 mM NaCl (T+NaCl). Hierarchical clustering by Euclidean distance method average linkage is shown. The color of the each cell indicates fold-change relative to control as follows: Red: significant up regulation ( P <0.05; t test), green: significant down regulation ( P <0.05; t test). Black: not statistically significant difference from the control,. (B) RNA was extracted from untreated cucumber seedlings (Control), from seedlings exposed to 100 mM NaCl (+NaCl), from seedlings colonized by T203 (+T) and from seedlings colonized by T203 for 48 hours prior exposure to 100 mM NaCl (+T+NaCl). RNA was extracted 1 day and 4 days after salt addition. Fold expression (for A and B) was calculated from average of three independent biological repetitions.

Article Snippet: DNaseI digestion was performed with the Turbo DNA-free Kit (Ambion). cDNA was prepared and hybridized to Agilent Arabidopsis (V4) Gene Expression Microarrays (4×44K) in the Microarrays Unit of the Weizmann Institute of Science (Rehovot, Israel), according to manufacturer instructions.

Techniques: Expressing

Journal: PLoS Pathogens

Article Title: Trichoderma -Plant Root Colonization: Escaping Early Plant Defense Responses and Activation of the Antioxidant Machinery for Saline Stress Tolerance

doi: 10.1371/journal.ppat.1003221

Figure Lengend Snippet: (A) Cucumber seeds were planted in untreated soil (Control) or in pots with soil mixed with a spore suspension (10 6 spores/g soil) of T203–WT, or ACC-deaminase silenced mutants ΔACC#2 and ΔACC#3. The pots were watered with either tap water or a solution of 75 mM NaCl. Germinating seedlings were counted 7–10 days after planting. (B) Arabidopsis seeds were planted as described for the cucumber seeds and watered with either tap water, 80 mM or 125 mM NaCl solution.

Article Snippet: DNaseI digestion was performed with the Turbo DNA-free Kit (Ambion). cDNA was prepared and hybridized to Agilent Arabidopsis (V4) Gene Expression Microarrays (4×44K) in the Microarrays Unit of the Weizmann Institute of Science (Rehovot, Israel), according to manufacturer instructions.

Techniques: